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exogenous recombinant mouse bmp4  (R&D Systems)


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    Structured Review

    R&D Systems exogenous recombinant mouse bmp4
    Exogenous Recombinant Mouse Bmp4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+recombinant+bmp4/Recombinant+Mouse+BMP-4+Protein/pm41820373-368-0-5
    Average 95 stars, based on 124 article reviews
    exogenous recombinant mouse bmp4 - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Isolation:

    Article Title: Sox9 drives columnar differentiation of esophageal squamous epithelium: a possible role in the pathogenesis of Barrett's esophagus.
    Article Snippet: Nicholas J. Clemons, David H. Wang, Daniel Croagh, Anjali Tikoo, Christina M. Fennell, Carmel Murone, Andrew M. Scott, D. Neil Watkins, and Wayne A. Phillips Surgical Oncology Research Laboratory, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia; Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, Victoria, Australia; Department of Surgery, St. Vincent’s Hospital, University of Melbourne, Melbourne, Victoria, Australia; Department of Medicine, Veterans Affairs North Texas Health Care System, University of Texas Southwestern Medical School, Dallas, Texas; Ludwig Institute for Cancer Research, Heidelberg, Victoria, Australia; and Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia

    Reconstitution Assay:

    Article Title: Sox9 drives columnar differentiation of esophageal squamous epithelium: a possible role in the pathogenesis of Barrett's esophagus.
    Article Snippet: Nicholas J. Clemons, David H. Wang, Daniel Croagh, Anjali Tikoo, Christina M. Fennell, Carmel Murone, Andrew M. Scott, D. Neil Watkins, and Wayne A. Phillips Surgical Oncology Research Laboratory, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia; Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, Victoria, Australia; Department of Surgery, St. Vincent’s Hospital, University of Melbourne, Melbourne, Victoria, Australia; Department of Medicine, Veterans Affairs North Texas Health Care System, University of Texas Southwestern Medical School, Dallas, Texas; Ludwig Institute for Cancer Research, Heidelberg, Victoria, Australia; and Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia

    Cell Culture:

    Article Title: Sox9 drives columnar differentiation of esophageal squamous epithelium: a possible role in the pathogenesis of Barrett's esophagus.
    Article Snippet: Nicholas J. Clemons, David H. Wang, Daniel Croagh, Anjali Tikoo, Christina M. Fennell, Carmel Murone, Andrew M. Scott, D. Neil Watkins, and Wayne A. Phillips Surgical Oncology Research Laboratory, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia; Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, Victoria, Australia; Department of Surgery, St. Vincent’s Hospital, University of Melbourne, Melbourne, Victoria, Australia; Department of Medicine, Veterans Affairs North Texas Health Care System, University of Texas Southwestern Medical School, Dallas, Texas; Ludwig Institute for Cancer Research, Heidelberg, Victoria, Australia; and Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia

    Retroviral:

    Article Title: Sox9 drives columnar differentiation of esophageal squamous epithelium: a possible role in the pathogenesis of Barrett's esophagus.
    Article Snippet: Nicholas J. Clemons, David H. Wang, Daniel Croagh, Anjali Tikoo, Christina M. Fennell, Carmel Murone, Andrew M. Scott, D. Neil Watkins, and Wayne A. Phillips Surgical Oncology Research Laboratory, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia; Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, Victoria, Australia; Department of Surgery, St. Vincent’s Hospital, University of Melbourne, Melbourne, Victoria, Australia; Department of Medicine, Veterans Affairs North Texas Health Care System, University of Texas Southwestern Medical School, Dallas, Texas; Ludwig Institute for Cancer Research, Heidelberg, Victoria, Australia; and Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia

    Transduction:

    Article Title: Sox9 drives columnar differentiation of esophageal squamous epithelium: a possible role in the pathogenesis of Barrett's esophagus.
    Article Snippet: Nicholas J. Clemons, David H. Wang, Daniel Croagh, Anjali Tikoo, Christina M. Fennell, Carmel Murone, Andrew M. Scott, D. Neil Watkins, and Wayne A. Phillips Surgical Oncology Research Laboratory, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia; Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, Victoria, Australia; Department of Surgery, St. Vincent’s Hospital, University of Melbourne, Melbourne, Victoria, Australia; Department of Medicine, Veterans Affairs North Texas Health Care System, University of Texas Southwestern Medical School, Dallas, Texas; Ludwig Institute for Cancer Research, Heidelberg, Victoria, Australia; and Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia

    Incubation:

    Article Title: Sox9 drives columnar differentiation of esophageal squamous epithelium: a possible role in the pathogenesis of Barrett's esophagus.
    Article Snippet: Nicholas J. Clemons, David H. Wang, Daniel Croagh, Anjali Tikoo, Christina M. Fennell, Carmel Murone, Andrew M. Scott, D. Neil Watkins, and Wayne A. Phillips Surgical Oncology Research Laboratory, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia; Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, Victoria, Australia; Department of Surgery, St. Vincent’s Hospital, University of Melbourne, Melbourne, Victoria, Australia; Department of Medicine, Veterans Affairs North Texas Health Care System, University of Texas Southwestern Medical School, Dallas, Texas; Ludwig Institute for Cancer Research, Heidelberg, Victoria, Australia; and Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia


    Article Title: BMP4 triggers regulatory circuits specifying the cardiac mesoderm lineage
    Article Snippet: .. Plates were then inverted and incubated for 24 h. Drops containing mESC aggregates [corresponding to differentiation day 0 or embryoid bodies stage (ES)] were then pooled, washed twice with DMEM (Thermo Fisher Scientific; 12491015) and resuspended in differentiation medium: 48% (v/v) DMEM/F12 (Thermo Fisher Scientific; 11320033), 48% (v/v) Neurobasal medium (Thermo Fisher Scientific; 21103049), 1% (v/v) 200 mM L-glutamine (Lonza; BE17-605E), 1% (v/v) penicillin and streptomycin, 1% (v/v) B27 (Gibco; 17504044), 0.5% (v/v) N2 (Gibco; 17502048), 0.5% (v/v) 7.5% bovine serum albumin (BSA) fraction V (Gibco; 15260037), 0.1 mM thioglycerol (Sigma-Aldrich; M1753) supplemented with 20 ng/ml mouse recombinant Bmp4 (R&D Systems; 5020-BP-010). .. To start differentiation, mESC aggregates resuspended in differentiation medium containing Bmp4 were immediately plated on fibronectin-coated plates (Calbiochem; 341631).

    Recombinant:

    Article Title: Sox9 drives columnar differentiation of esophageal squamous epithelium: a possible role in the pathogenesis of Barrett's esophagus.
    Article Snippet: Nicholas J. Clemons, David H. Wang, Daniel Croagh, Anjali Tikoo, Christina M. Fennell, Carmel Murone, Andrew M. Scott, D. Neil Watkins, and Wayne A. Phillips Surgical Oncology Research Laboratory, Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia; Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne, Victoria, Australia; Department of Surgery, St. Vincent’s Hospital, University of Melbourne, Melbourne, Victoria, Australia; Department of Medicine, Veterans Affairs North Texas Health Care System, University of Texas Southwestern Medical School, Dallas, Texas; Ludwig Institute for Cancer Research, Heidelberg, Victoria, Australia; and Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia

    Article Title: FGF and BMP signaling are required for specifying pre-chondrogenic identity in neural crest derived mesenchyme and initiating the chondrogenic program
    Article Snippet: Bead implantation Affi-Gel Blue Beads (100-200 μm, Bio-Rad, Hercules, CA) were washed in PBS for 1 hour. .. Beads were then soaked in 10 mM SU5402 (Pfizer), or 1 mg/ml human recombinant FGF8 (423-F8/CF, R&D systems), FGF19 (100-32, Peprotech), or 100 ng/ml mouse recombinant BMP4 (314-BP/CF, R&D systems) for 1 hour. ..

    Article Title: Hyaluronic acid-CD44 interactions promote BMP4/7-dependent Id1/3 expression in melanoma cells
    Article Snippet: .. Stimulation of tumor cells with mouse recombinant BMP4 or BMP7 (R&D Systems, Wiesbaden-Nordenstadt, Germany) was performed with the indicated concentrations. .. Bovine testis hyaluronidase (BTH) and 4-MU sodium salt were purchased from Sigma-Aldrich, Taufkirchen, Germany.


    Article Title: BMP4 triggers regulatory circuits specifying the cardiac mesoderm lineage
    Article Snippet: .. Plates were then inverted and incubated for 24 h. Drops containing mESC aggregates [corresponding to differentiation day 0 or embryoid bodies stage (ES)] were then pooled, washed twice with DMEM (Thermo Fisher Scientific; 12491015) and resuspended in differentiation medium: 48% (v/v) DMEM/F12 (Thermo Fisher Scientific; 11320033), 48% (v/v) Neurobasal medium (Thermo Fisher Scientific; 21103049), 1% (v/v) 200 mM L-glutamine (Lonza; BE17-605E), 1% (v/v) penicillin and streptomycin, 1% (v/v) B27 (Gibco; 17504044), 0.5% (v/v) N2 (Gibco; 17502048), 0.5% (v/v) 7.5% bovine serum albumin (BSA) fraction V (Gibco; 15260037), 0.1 mM thioglycerol (Sigma-Aldrich; M1753) supplemented with 20 ng/ml mouse recombinant Bmp4 (R&D Systems; 5020-BP-010). .. To start differentiation, mESC aggregates resuspended in differentiation medium containing Bmp4 were immediately plated on fibronectin-coated plates (Calbiochem; 341631).

    other:

    Article Title: R-Smad signaling-mediated VEGF expression coordinately regulates endothelial cell differentiation of rat mesenchymal stem cells.
    Article Snippet: A low-efficiency yield hinders the use of stem cells as a source of endothelial cells (ECs) for therapeutic vascularization, and the diversity of the transforming growth factor-β (TGF-β) Page 1 of 38 St em C el ls a nd D ev el op m en t R -S m ad S ig na lin gm ed ia te d V E G F E xp re ss io n C oo rd in at el y R eg ul at es E nd ot he lia l C el l D if fe re nt ia tio n of R at M es en ch ym al S te m C el ls ( do i: 10 .1 08 9/ sc d. 20 14 .0 25 3) T hi s ar tic le h as b ee n pe er -r ev ie w ed a nd a cc ep te d fo r pu bl ic at io n, b ut h as y et to u nd er go c op ye di tin g an d pr oo f co rr ec tio n. T he f in al p ub lis he d ve rs io n m ay d if fe r fr om th is p ro of . 2 superfamily has undermined understanding the effects of its potent vascularization-inducing.. Herein, we studied the role of the TGF-β superfamily in EC differentiation of rat bone marrow mesenchymal stem cells (MSCs) induced by Smad2/3 and Smad1/5/8 signaling.. MSCs that had been sorted by flow cytometry as CD31-negative were cultured for 14 days in medium supplemented with vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) as the control.



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    ( A ) Volcano plot of differentially accessible chromatin sites after 8 hours of YAP CA expression. Log 2 fold change > 2 indicates more accessible chromatin after YAP CA expression. ( B ) Motif analysis on differentially accessible chromatin loci. ( C ) Chromatin accessibility at locus proximate to Ctgf . ( D ) Chromatin immunoprecipitation of CTGF by YAP and TEAD in human cells. ( E ) Chromatin accessibility at locus proximate to <t>Bmp4</t> . ( F ) Chromatin immunoprecipitation of BMP4 by YAP and TEAD in human cells. ( G ) Temporal dynamics of Yap and Bmp4 expression after 1, 4, 8, 12, 24, and 48 hours of doxycycline treatment. ( H ) Schematic for femoral osteotomies and periosteal cell isolation followed by MGH-CP1 treatment in vitro. ( I ) Bmp4 mRNA expression after 24 hours of treatment with 10 µm or 50 µm of MGH-CP1 in vitro. ( J ) Coimmunoprecipitation of TEAD with YAP in fracture-activated periosteal cells. IP, immunoprecipitation; IB, immunoblotting. ( K ) RNA in situ hybridization for Bmp4 in the periosteum of WT fl/fl and YAP/TAZcKO Osx mice. ( L ) Staining for Osx protein and DAPI in adjacent sections to sections in (K). Dotted lines indicate the periosteum. Scale bars, 100 μm. ** P < 0.01 and **** P < 0.0001.
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    Image Search Results


    ( A ) Volcano plot of differentially accessible chromatin sites after 8 hours of YAP CA expression. Log 2 fold change > 2 indicates more accessible chromatin after YAP CA expression. ( B ) Motif analysis on differentially accessible chromatin loci. ( C ) Chromatin accessibility at locus proximate to Ctgf . ( D ) Chromatin immunoprecipitation of CTGF by YAP and TEAD in human cells. ( E ) Chromatin accessibility at locus proximate to Bmp4 . ( F ) Chromatin immunoprecipitation of BMP4 by YAP and TEAD in human cells. ( G ) Temporal dynamics of Yap and Bmp4 expression after 1, 4, 8, 12, 24, and 48 hours of doxycycline treatment. ( H ) Schematic for femoral osteotomies and periosteal cell isolation followed by MGH-CP1 treatment in vitro. ( I ) Bmp4 mRNA expression after 24 hours of treatment with 10 µm or 50 µm of MGH-CP1 in vitro. ( J ) Coimmunoprecipitation of TEAD with YAP in fracture-activated periosteal cells. IP, immunoprecipitation; IB, immunoblotting. ( K ) RNA in situ hybridization for Bmp4 in the periosteum of WT fl/fl and YAP/TAZcKO Osx mice. ( L ) Staining for Osx protein and DAPI in adjacent sections to sections in (K). Dotted lines indicate the periosteum. Scale bars, 100 μm. ** P < 0.01 and **** P < 0.0001.

    Journal: Science Advances

    Article Title: YAP regulates transcriptional programs for layer-specific periosteal expansion during fracture repair

    doi: 10.1126/sciadv.adw0126

    Figure Lengend Snippet: ( A ) Volcano plot of differentially accessible chromatin sites after 8 hours of YAP CA expression. Log 2 fold change > 2 indicates more accessible chromatin after YAP CA expression. ( B ) Motif analysis on differentially accessible chromatin loci. ( C ) Chromatin accessibility at locus proximate to Ctgf . ( D ) Chromatin immunoprecipitation of CTGF by YAP and TEAD in human cells. ( E ) Chromatin accessibility at locus proximate to Bmp4 . ( F ) Chromatin immunoprecipitation of BMP4 by YAP and TEAD in human cells. ( G ) Temporal dynamics of Yap and Bmp4 expression after 1, 4, 8, 12, 24, and 48 hours of doxycycline treatment. ( H ) Schematic for femoral osteotomies and periosteal cell isolation followed by MGH-CP1 treatment in vitro. ( I ) Bmp4 mRNA expression after 24 hours of treatment with 10 µm or 50 µm of MGH-CP1 in vitro. ( J ) Coimmunoprecipitation of TEAD with YAP in fracture-activated periosteal cells. IP, immunoprecipitation; IB, immunoblotting. ( K ) RNA in situ hybridization for Bmp4 in the periosteum of WT fl/fl and YAP/TAZcKO Osx mice. ( L ) Staining for Osx protein and DAPI in adjacent sections to sections in (K). Dotted lines indicate the periosteum. Scale bars, 100 μm. ** P < 0.01 and **** P < 0.0001.

    Article Snippet: Recombinant mouse BMP4 (cat. no. 5020-BP, R&D Systems, Minneapolis, MN) was reconstituted in 0.9% sterile saline solution (cat. no. S5825, Teknova, Hollister, CA) at 12.5 μg/ml.

    Techniques: Expressing, Chromatin Immunoprecipitation, Cell Isolation, In Vitro, Immunoprecipitation, Western Blot, RNA In Situ Hybridization, Staining

    ( A ) Timeline of in vivo recombinant mouse BMP4 (rmBMP4) delivery for 4 days after fracture in YAP/TAZ cKO Osx mice. ( B ) Immunofluorescence staining of Osx protein with DAPI and EdU. Dotted lines indicate the periosteum and the separation between the Osx + cambium and Osx − fibrous layers. ( C ) Average periosteal thickness. ( D ) Percentage of proliferative EdU + cells in the periosteum. ( E ) Quantification of cambium and fibrous layer thickness. ( F ) Percentage of proliferative EdU + cells in each layer. Scale bars, 100 μm. * P < 0.05, ** P < 0.01.

    Journal: Science Advances

    Article Title: YAP regulates transcriptional programs for layer-specific periosteal expansion during fracture repair

    doi: 10.1126/sciadv.adw0126

    Figure Lengend Snippet: ( A ) Timeline of in vivo recombinant mouse BMP4 (rmBMP4) delivery for 4 days after fracture in YAP/TAZ cKO Osx mice. ( B ) Immunofluorescence staining of Osx protein with DAPI and EdU. Dotted lines indicate the periosteum and the separation between the Osx + cambium and Osx − fibrous layers. ( C ) Average periosteal thickness. ( D ) Percentage of proliferative EdU + cells in the periosteum. ( E ) Quantification of cambium and fibrous layer thickness. ( F ) Percentage of proliferative EdU + cells in each layer. Scale bars, 100 μm. * P < 0.05, ** P < 0.01.

    Article Snippet: Recombinant mouse BMP4 (cat. no. 5020-BP, R&D Systems, Minneapolis, MN) was reconstituted in 0.9% sterile saline solution (cat. no. S5825, Teknova, Hollister, CA) at 12.5 μg/ml.

    Techniques: In Vivo, Recombinant, Immunofluorescence, Staining

    ( A ) ALP activity in the periosteum of YAP/TAZ cKO Osx mice treated with saline or BMP4 at 4 DPF. ( B and C ) Quantification of ALP intensity (B) and ALP + area fraction (C). ( D ) Blood vessels marked by endomucin (Emcn) staining. ( E ) Frequency distribution of blood vessel loop size. ( F ) SHG imaging of collagen matrix content and organization. ( G ) Quantification of SHG signal. Scale bars, 100 μm. * P < 0.05.

    Journal: Science Advances

    Article Title: YAP regulates transcriptional programs for layer-specific periosteal expansion during fracture repair

    doi: 10.1126/sciadv.adw0126

    Figure Lengend Snippet: ( A ) ALP activity in the periosteum of YAP/TAZ cKO Osx mice treated with saline or BMP4 at 4 DPF. ( B and C ) Quantification of ALP intensity (B) and ALP + area fraction (C). ( D ) Blood vessels marked by endomucin (Emcn) staining. ( E ) Frequency distribution of blood vessel loop size. ( F ) SHG imaging of collagen matrix content and organization. ( G ) Quantification of SHG signal. Scale bars, 100 μm. * P < 0.05.

    Article Snippet: Recombinant mouse BMP4 (cat. no. 5020-BP, R&D Systems, Minneapolis, MN) was reconstituted in 0.9% sterile saline solution (cat. no. S5825, Teknova, Hollister, CA) at 12.5 μg/ml.

    Techniques: Activity Assay, Saline, Staining, Imaging

    ( A ) Timeline of in vivo recombinant mouse BMP4 delivery for 4 days after fracture in WT YAP/TAZ fl/fl mice. ( B ) Immunofluorescence staining of Osx protein with DAPI and EdU. Dotted lines indicate the periosteum and the separation between the Osx + cambium and Osx − fibrous layers. ( C ) Average periosteal thickness. ( D ) Percentage of proliferative EdU + cells in the periosteum. ( E ) Quantification of cambium and fibrous layer thickness. ( F ) Percentage of proliferative EdU + cells in each layer. Scale bars, 100 μm.

    Journal: Science Advances

    Article Title: YAP regulates transcriptional programs for layer-specific periosteal expansion during fracture repair

    doi: 10.1126/sciadv.adw0126

    Figure Lengend Snippet: ( A ) Timeline of in vivo recombinant mouse BMP4 delivery for 4 days after fracture in WT YAP/TAZ fl/fl mice. ( B ) Immunofluorescence staining of Osx protein with DAPI and EdU. Dotted lines indicate the periosteum and the separation between the Osx + cambium and Osx − fibrous layers. ( C ) Average periosteal thickness. ( D ) Percentage of proliferative EdU + cells in the periosteum. ( E ) Quantification of cambium and fibrous layer thickness. ( F ) Percentage of proliferative EdU + cells in each layer. Scale bars, 100 μm.

    Article Snippet: Recombinant mouse BMP4 (cat. no. 5020-BP, R&D Systems, Minneapolis, MN) was reconstituted in 0.9% sterile saline solution (cat. no. S5825, Teknova, Hollister, CA) at 12.5 μg/ml.

    Techniques: In Vivo, Recombinant, Immunofluorescence, Staining

    ( A ) ALP activity in the periosteum of YAP fl/fl TAZ fl/fl mice treated with saline or BMP4 at 4 DPF. ( B and C ) Quantification of ALP intensity (B) and ALP + area fraction (C). ( D ) Blood vessels marked by endomucin staining. ( E ) Frequency distribution of blood vessel loop size. K-S, Kolmogorov-Smirnov. ( F ) SHG imaging of collagen matrix content and organization. ( G ) Quantification of SHG signal. Scale bars, 100 μm. * P < 0.05, ** P < 0.01.

    Journal: Science Advances

    Article Title: YAP regulates transcriptional programs for layer-specific periosteal expansion during fracture repair

    doi: 10.1126/sciadv.adw0126

    Figure Lengend Snippet: ( A ) ALP activity in the periosteum of YAP fl/fl TAZ fl/fl mice treated with saline or BMP4 at 4 DPF. ( B and C ) Quantification of ALP intensity (B) and ALP + area fraction (C). ( D ) Blood vessels marked by endomucin staining. ( E ) Frequency distribution of blood vessel loop size. K-S, Kolmogorov-Smirnov. ( F ) SHG imaging of collagen matrix content and organization. ( G ) Quantification of SHG signal. Scale bars, 100 μm. * P < 0.05, ** P < 0.01.

    Article Snippet: Recombinant mouse BMP4 (cat. no. 5020-BP, R&D Systems, Minneapolis, MN) was reconstituted in 0.9% sterile saline solution (cat. no. S5825, Teknova, Hollister, CA) at 12.5 μg/ml.

    Techniques: Activity Assay, Saline, Staining, Imaging